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For total persufide measurement, 100 L samples were reacted with 20 mM TCEP for 3 min and then mixed with 40 mM IAM and further incubated at RT in the dark for 30 min

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The isolated RNA was quantified spectrophotometrically using the A260/A280 ratio

Tissues were permeabilized with PBS supplemented with 0.1% Tween-20 for 10 min, followed by a peroxidase block with 3% H 2 O 2 (Sigma-Aldrich, 216763) for 20 min

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