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Activity measurement was based on the decrease of orotate absorbance at 290 nm as it is converted to orotidine monophosphate by the OPRT enzyme
The Hp1/2 proteins form different oligomers, such that a dimeric Hp11 molecule can bind two haemoglobin dimers, while the polymeric Hp21 and Hp 22 molecules have the capacity to bind greater numbers of haemoglobin dimers (Morton et al., 2006)
10.1016/j.celrep.2020.02.049 44 FigueiredoC
As a methyl donor, SAM participates in various methyl transfer reactions and is converted to S-adenosylhomocysteine (SAH) in the reaction
